ABSTRACT:- Gel permeation chromatography (GPC) uses an eluent (mobile phase) and a porous gel that separates polymer molecules based on size or hydrodynamic volume. Smaller molecules spend more time in the pores, which increases retention time. A common eluent is tetrahydrofuran. GPC is used to determine relative molecular weights of polymers. Thus, the pore size of the gel is controlled. Grubisic, et al (1967) reasoned that the Einstein viscosity law, [ η ] = K ( Ve/M ), could be employed to allow for a universal correlation. [ η ] is the intrinsic viscosity, Ve is the hydrodynamic volume of the polymer, M is the molecular weight of the polymer and K is a constant. Flory (1953) says K = 0.025 No, where No is Avogadro’s number. We present the graph from Grubisic, et al as reproduced in Bovey/Winslow (1979) with comments and how this is useful for polymer science.

KEYWORDS:- “gel permeation chrmatography” “hydrodynamic volume” “Einstein viscosity law”